arrayexpress e mtab 5305 mouse embryo single cell rna seq data deng (ATCC)
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arrayexpress e mtab 5305 mouse embryo single cell rna seq data deng
Arrayexpress E Mtab 5305 Mouse Embryo Single Cell Rna Seq Data Deng, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arrayexpress+e+mtab+5305+mouse+embryo+single+cell+rna+seq+data+deng/Mouse+Total+RNA/pm41385374-894-222-242
Average 95 stars, based on 117 article reviews
Arrayexpress E Mtab 5305 Mouse Embryo Single Cell Rna Seq Data Deng, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arrayexpress+e+mtab+5305+mouse+embryo+single+cell+rna+seq+data+deng/Mouse+Total+RNA/pm41385374-894-222-242
Average 95 stars, based on 117 article reviews
arrayexpress e mtab 5305 mouse embryo single cell rna seq data deng - by Bioz Stars,
2026-09
95/100 stars
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SYBR Green Assay:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Real-time Polymerase Chain Reaction:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Transfection:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: XF Assay:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Cell Recovery:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Activity Assay:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Western Blot:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Bicinchoninic Acid Protein Assay:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Polymerase Chain Reaction:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Purification:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Qubit Protein Assay:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Clone Assay:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: RNA Sequencing:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Quantitative RT-PCR:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: Software:Article Title: The exit of naive pluripotency contains a metabolism-induced checkpoint for telomere homeostasis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStart SYBR Green qPCR ReadyMix Sigma-Aldrich KCQS02 Lipofectamine transfection reagent Thermo Fisher Scientific 100014469 TelC-Cy3 probe PNA Bio F1002 Seahorse XF Assay Medium Agilent 103575 Matrigel Cell Recovery solution Corning 354253 N-2 Supplement (100×) Gibco 17502048 Critical commercial assays Alkaline Phosphatase activity Sigma-Aldrich 86R-1KT Chromium Next GEM Single Cell 3 ′ GEM, Library & Gel Bead Kit v3.1 10× Genomics PN-1000121 Clarity Western ECL Substrate Bio-Rad 1705061 DNeasy Blood & Tissue Kit Qiagen 69504 eBioscience AnnexinV-FITC Apoptosis Detection Kit Thermo Fisher Scientific BMS500Fi NextSeq 500/550 High Output Kit v2.5 Illumina 20024906 MycoAlert Mycoplasma Detection Kit Lonza LT07-118 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 QIAquick PCR Purification Kit Qiagen 28106 Qubit Protein Assay Kit Thermo Fisher Scientific Q33211 Qubit 1× dsDNA HS assay kit Thermo Fisher Scientific Q33231 RNeasy Micro Kit Qiagen 74004 RNeasy Mini Kit Qiagen 74106 Seahorse Fluxpak Agilent 102601-100 Deposited data ESC LD clones bulk RNA-seq data This paper GEO: GSE270063; SuperSeries GEO: GSE271241 ESC LD clones single-cell RNA-seq data This paper GEO: GSE253432; SuperSeries GEO: GSE271241 ESC-derived 3D spheroids bulk RNA-seq data Mau et al. 28 GEO: GSE165563 ESC and EpiLC bulk RNA-seq data Buecker et al. 41 GEO: GSE56138 ESC and EpiSC bulk RNA-seq data Factor et al. 40 GEO: GSE57409 Rex1-GFP ESC bulk RNA-seq data Kalkan et al. 11 ArrayExpress: E-MTAB-5305 Mouse embryo single-cell RNA-seq data Deng et al. 69 GEO: GSE45719 Experimental models: |